Application

Research CategoryStem Cell Research

Cell Line Description

Neural Stem Cells

Components

> 1x10⁶ viable ReNcell VM cells derived from human ventral mesencephalon brain tissue, cryopreserved. Store in liquid nitrogen.

General description

Product Source: Human ventral mesencephalon brain tissue

ReNcell VM is an immortalized human neural progenitor cell line with the ability to readily differentiate into neurons and glial cells. ReNcell VM was derived from the ventral mesencephalon region of human fetal brain. Immortalized by retroviral transduction with the v-myc oncogene, this cell line grows rapidly as a monolayer on laminin with a doubling time of 20-30 hours. Karyotype analyses indicate that the ReNcell VM retains a normal diploid karyotype in culture even after prolonged passage (>45 passages). ReNcell VM was developed by the ReNeuron Group plc, a biotech company that specializes in using human somatic stem cells for therapeutics. In experiments performed by the ReNeuron Group plc, ReNcell VM can be differentiated in vitro to a high level of human dopaminergic neurons. Neurons differentiated from ReNcell VM have furthermore been shown to be electophysiologically active. ReNcell VM may be used for a variety of research applications such as studies of neurotoxicity, neurogenesis, electrophysiology, neurotransmitter and receptor functions. ReNcell VM cells have been obtained in a legal and ethical manner, compliant with current local informed consent procedures.ReNcell Now Used in a 3D Cell Model of Alzheimer′s Disease! Tanzi, R and Kim, DY et. al. A three-dimensional human neural cell culture model of Alzheimer′s disease. Nature. 2014 Nov 13;515(7526):274-8.(Published in Nature)

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

This product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the 'Academic Use Agreement' as detailed in the product documentation. For information regarding any other use, please contact [email protected].

Quality

Each lot of ReNcell VM cells has been validated for high level of expression of Nestin and Sox 2 and for their self-renewal and multi-lineage differentiation capacities. Cells also display normal karyotype as assessed by chromosome spread and tested negative for mycoplasma. Maternal blood has been screened for HIV, HTLV, Hepatitis B and C.

Storage and Stability

When stored at the recommended storage conditions (liquid nitrogen), ReNcell VM cells are stable up to the expiration date. Do not expose to elevated temperatures. Discard any remaining reagents after the expiration date. We recommend that the cells be used within ten passages.